Crystal Structure and Evolution of a Prokaryotic Glucoamylase
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چکیده
منابع مشابه
Crystal structure and evolution of a prokaryotic glucoamylase.
The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eu...
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In this study, the x-ray crystal structures of the calcium-free and calcium-bound forms of phospholipase A(2) (PLA(2)), produced extracellularly by Streptomyces violaceoruber, were determined by using the multiple isomorphous replacement and molecular replacement methods, respectively. The former and latter structures were refined to an R-factor of 18.8% at a 1.4-A resolution and an R-factor of...
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B acterial cytoplasmic membranes consist primarily of amphipathic phospholipids with lesser amounts of glycolipids, providing a degree of protection from deleterious, toxic substances that is lacking in organisms having a single membrane. Almost all bacterial membranes are assembled in bilayers with embedded integral and associated peripheral membrane proteins. In the case of gram-negative bact...
متن کاملCrystal structure of a Kir3.1-prokaryotic Kir channel chimera.
The Kir3.1 K(+) channel participates in heart rate control and neuronal excitability through G-protein and lipid signaling pathways. Expression in Escherichia coli has been achieved by replacing three fourths of the transmembrane pore with the pore of a prokaryotic Kir channel, leaving the cytoplasmic pore and membrane interfacial regions of Kir3.1 origin. Two structures were determined at 2.2 ...
متن کاملSYNTHESIS AND CRYSTAL STRUCTURE OF [Pph ] [WS (CUNCS) ] A. Taeb A. Beheshti and N.K. Javadi SYNTHESIS AND CRYSTAL STRUCTURE OF [Pph ] [WS (CUNCS) ]
The crystals of [WS (CuNCS) ] anion have been isolated from CH ,CI solution and examined by X-ray structural analysis as well as by IR spectroscopy. The unit cell is monoclinic with a= 14.3796(13), b= 18.039(1 I), c= 22.5956(23)& P= 96.053(8)', space group P2,/n (14), Z= 4. The crystal structure was refined by the least-squares calculations to a final R-value of 0.0398(Rw= 0.0443) for 2777 u...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2003
ISSN: 0022-2836
DOI: 10.1016/s0022-2836(03)00084-6